MEASUREMENT OF PROSTATIC ACID PHOSPHATASE BY GEL DIFFUSION METHODS
نویسندگان
چکیده
منابع مشابه
Diffusion measurement in ferrous infused gel dosimeters
Background: The compositions of Ferrous sulphate, Agarose and Xylenol orange dye (FAX) and Ferrous sulphate, Gelatin and Xylenol orange dye (FGX) in solution of distilled water and sulphuric acid are two tissue-equivalent gel dosimeters. Ionizing radiation causes oxidation of Fe2+ ion to Fe3+ ions which diffuse through the gel matrix and blur the image of absorbed dose over a period of hours af...
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In 1938, Gutman et al. (6) described increased levels of acid phosphatase in metastatic carcinoma of the prostate. Since then, when most of us hear the term "acid phosphatase" we immediately think of prostatic cancer, and justifiably so. The link, however, between the test and the diagnosis is not as clear and simple as many of us would like to believe. I would first like to discuss this enzyme...
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We developed an assay to measure at acid pH the phosphotyrosine phosphatase activity in sera from patients with prostatic cancer. The method used quantifies the inorganic phosphate liberated from phosphotyrosine after incubation with serum, followed by the deproteinization of the reaction mixture. A high acid phosphatase (EC 3.1.3.2) activity towards phosphotyrosine was observed in all sera fro...
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We quantitated the concentrations of prostatic acid phosphatases (EC 3.1.3.2) in serum and bone-marrow aspirates with three commercial radioimmunoassay kits, and the catalytic activities with a thymolphthalein monophosphate-based enzyme test. The enzyme's immunological activity in serum was compared with its catalytic activity for its potential as a detector of early prostatic cancer and its pe...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1965
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0100055